An alternative strategy to generate coding sequence of macrophage migration inhibitory factor-2 of Wuchereria bancrofti.
Identifieur interne : 001433 ( Main/Exploration ); précédent : 001432; suivant : 001434An alternative strategy to generate coding sequence of macrophage migration inhibitory factor-2 of Wuchereria bancrofti.
Auteurs : Nikhil Chauhan ; S L Hoti [Inde]Source :
- The Indian journal of medical research [ 0971-5916 ] ; 2016.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN complémentaire (isolement et purification), Animaux, Exons (génétique), Facteurs inhibiteurs de la migration des macrophages (génétique), Facteurs inhibiteurs de la migration des macrophages (isolement et purification), Filariose lymphatique (diagnostic), Filariose lymphatique (génétique), Filariose lymphatique (parasitologie), Humains, Réaction de polymérisation en chaîne, Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification), Wuchereria bancrofti (pathogénicité).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : ADN complémentaire, Exons, Facteurs inhibiteurs de la migration des macrophages, Filariose lymphatique, Wuchereria bancrofti.
- isolement et purification : ADN complémentaire, Facteurs inhibiteurs de la migration des macrophages, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- pathogénicité : Wuchereria bancrofti.
- Animaux, Humains, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- Animals, DNA, Complementary (genetics), DNA, Complementary (isolation & purification), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (genetics), Elephantiasis, Filarial (parasitology), Exons (genetics), Humans, Macrophage Migration-Inhibitory Factors (genetics), Macrophage Migration-Inhibitory Factors (isolation & purification), Polymerase Chain Reaction, Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification), Wuchereria bancrofti (pathogenicity).
- MESH :
- chemical , genetics : DNA, Complementary, Macrophage Migration-Inhibitory Factors.
- chemical , isolation & purification : DNA, Complementary, Macrophage Migration-Inhibitory Factors.
- diagnosis : Elephantiasis, Filarial.
- genetics : Elephantiasis, Filarial, Exons, Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- pathogenicity : Wuchereria bancrofti.
- Animals, Humans, Polymerase Chain Reaction.
Abstract
Different developmental stages of Wuchereria bancrofti, the major causal organism of lymphatic filariasis (LF), are difficult to obtain. Beside this limitation, to obtain complete coding sequence (CDS) of a gene one has to isolate mRNA and perform subsequent cDNA synthesis which is laborious and not successful at times. In this study, an alternative strategy employing polymerase chain reaction (PCR) was optimized and validated, to generate CDS of Macrophage migration Inhibitory Factor-2 (wbMIF-2), a gene expressed in the transition stage between L3 to L4.
DOI: 10.4103/0971-5916.180217
PubMed: 27121522
Affiliations:
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Le document en format XML
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Scientist 'G' and Director-in-Charge, Regional Medical Research Centre (ICMR), Nehru Nagar, Belagavi 590 010, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Microbiology & Immunology, Vector Control Research Centre (ICMR), Puducherry; Scientist 'G' and Director-in-Charge, Regional Medical Research Centre (ICMR), Nehru Nagar, Belagavi 590 010, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Indian journal of medical research</title><idno type="ISSN">0971-5916</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (isolation & purification)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (genetics)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Exons (genetics)</term><term>Humans</term><term>Macrophage Migration-Inhibitory Factors (genetics)</term><term>Macrophage Migration-Inhibitory Factors (isolation & purification)</term><term>Polymerase Chain Reaction</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term><term>Wuchereria bancrofti (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN complémentaire (isolement et purification)</term><term>Animaux</term><term>Exons (génétique)</term><term>Facteurs inhibiteurs de la migration des macrophages (génétique)</term><term>Facteurs inhibiteurs de la migration des macrophages (isolement et purification)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (génétique)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (isolement et purification)</term><term>Wuchereria bancrofti (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Macrophage Migration-Inhibitory Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term><term>Macrophage Migration-Inhibitory Factors</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Exons</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Exons</term><term>Facteurs inhibiteurs de la migration des macrophages</term><term>Filariose lymphatique</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term><term>Facteurs inhibiteurs de la migration des macrophages</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Different developmental stages of Wuchereria bancrofti, the major causal organism of lymphatic filariasis (LF), are difficult to obtain. Beside this limitation, to obtain complete coding sequence (CDS) of a gene one has to isolate mRNA and perform subsequent cDNA synthesis which is laborious and not successful at times. In this study, an alternative strategy employing polymerase chain reaction (PCR) was optimized and validated, to generate CDS of Macrophage migration Inhibitory Factor-2 (wbMIF-2), a gene expressed in the transition stage between L3 to L4.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Chauhan, Nikhil" sort="Chauhan, Nikhil" uniqKey="Chauhan N" first="Nikhil" last="Chauhan">Nikhil Chauhan</name></noCountry><country name="Inde"><noRegion><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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